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Send InquiryHPLC High Pressure Liquid Chromatography
HPLC High Pressure Liquid Chromatography Specification
- Features
- High-pressure pump (up to 6000 psi), auto-sampler, degasser, user-friendly LCD touch screen, multi-solvent delivery, data acquisition software
- Spare Parts
- Columns, seals, piston, injector, and tubing
- Theory
- Used for separating, identifying, and quantifying components in a mixture based on their different rates of distribution between a stationary phase and a mobile phase under high pressure.
- Sensor
- UV/Vis detector, PDA (photodiode array) detector, RI (refractive index) detector options available
- Resolution
- Highly sensitive; typically in the range of nanograms (ng)
- Interface
- RS232, USB, LAN connectivity
- Dimensions
- Approx. 480 mm (W) × 460 mm (D) × 600 mm (H)
- Working Stage
- Stainless steel column compartment
- Light Source
- UV lamp for detector
- Pump Flow Rate Range
- 0.01 - 10 mL/min
- Number of Channels
- Up to 4 solvent inlets
- Detector Type
- UV-Visible, PDA, or RI as per model
- Pressure Range
- 0 - 6000 psi (approx. 400 bar)
- Column Oven Temperature Control
- Ambient to 65C
- Weight
- Approx. 45 kg
- Compliance
- GLP/GMP compatible
- Power Supply
- 220 V, 50/60 Hz
- Sample Injection Volume
- 1 L to 2000 L (auto-sampler)
HPLC High Pressure Liquid Chromatography About
HPLC High Pressure Liquid Chromatography is a technique of separating mixture of sample and analyzing quantity of each component. It employs use of column with a stationary phase & mobile phase. This HPLC system is completely reliable in nature and assures accurate results. It is most commonly used for analytical chemistry applications. HPLC High Pressure Liquid Chromatography system comprises a solvent reservoir, high pressure pump, detector, & injector system.
Features:
- Hassle-free to use
- Completely trustworthy in nature
- Easy to maintain
Precision Engineering for Versatile Applications
Designed for proficient separation and analysis, this HPLC system integrates high-pressure multichannel pumping, advanced detectors, and robust temperature control. Users can manage up to four different solvents to facilitate gradient elution, ensuring highly sensitive and accurate results for a wide array of sample types.
High Sensitivity and Efficient Workflow
With detection capabilities down to the nanogram level, the system delivers exceptional resolution and quantitative performance. The intuitive LCD touch screen and data acquisition software streamline setup and operation, while auto-sampling and temperature regulation help achieve reproducible chromatographic outcomes.
Enduring Reliability and Regulatory Compliance
Built with a durable stainless steel column compartment and supported by comprehensive spare parts availability, the system is GLP/GMP compliant to meet regulatory demands in pharmaceutical and quality assurance labs. Compatible with multiple data interfaces, it is primed for integration and reliable data management.
FAQs of HPLC High Pressure Liquid Chromatography:
Q: How does this HPLC system ensure precise and reproducible results?
A: The system incorporates a high-pressure pump capable of delivering consistent flow rates within 0.01 to 10 mL/min and features stable column temperature control (ambient to 65C). These parameters, combined with autosampler precision (12000 L), facilitate reproducible injections and accurate separation of components.Q: What detector options are available, and how do I choose the right type for my analysis?
A: Detector choices include UV-Visible, Photodiode Array (PDA), and Refractive Index (RI). UV-Visible and PDA detectors are ideal for analytes absorbing in the UV-visible range. PDA offers spectral scanning, while RI is suitable for compounds without significant UV absorbance. Selection depends on the chemical properties of your target analytes.Q: When should the auto-sampler be used, and what injection volumes are supported?
A: The integrated auto-sampler is recommended for high-throughput laboratories to automate sample injections, reducing manual errors and increasing efficiency. It supports injection volumes ranging from 1 L to 2000 L, catering to both micro and semi-preparative applications.Q: Where can this HPLC system be installed and who are the ideal users?
A: This system is suitable for installation in research institutes, pharmaceutical quality control and R&D labs, food safety testing centers, and environmental monitoring facilities. It is designed for professionals needing fast, sensitive, and reliable chromatographic analysis.Q: What is the process for solvent management with up to four inlets?
A: The systems multi-channel pump allows connection of up to four different solvent bottles. Gradient elution can be programmed via the LCD touch interface to deliver precise solvent mixing and flow, optimizing the separation process for complex samples.Q: How does the system benefit users in regulated environments?
A: Being GLP/GMP compliant, the HPLC supports documentation, validation, and audit trails essential for regulated industries. Data acquisition software and secure connectivity (RS232, USB, LAN) facilitate traceable and compliant data management.Q: What spare parts are readily available, and how does that support ongoing maintenance?
A: To ensure minimal downtime and extended service life, spare parts such as columns, seals, pistons, injectors, and tubing are readily available through authorized distributors and suppliers across India, facilitating prompt maintenance or repairs as needed.
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