High Performance Thin Layer Chromatography (HPTLC)

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High Performance Thin Layer Chromatography (HPTLC)

Price 6000000.0 INR/ Number

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MOQ : 1 Number

High Performance Thin Layer Chromatography (HPTLC) Specification

Spare Parts
UV tubes, scanning module, plate carriers, lamps, application syringe, sample trays

Focus System
Automated and manual focusing for precise zone detection

Features
Automated sample application, programmable development chambers, high-throughput analysis, integrated densitometric quantification, multi-wavelength detection, easy data management.

Theory
Other

Sensor
High sensitivity densitometric scanner (integrated or standalone device)

Resolution
Up to 1 ng per band detection limit, enhanced zone resolution due to fine particle size on HPTLC plates.

Interface
RS232C/USB for data transfer to computer systems

Focal Distance
Adjustable, optimized for plate surface and scanner alignment Micromete (micron)

Magnification
Not applicable (zone or spot detection by scanner rather than optical magnification)

Dimensions
Depends on manufacturer, typically 60 x 30 x 30 cm (scanner unit) Micromete (micron)

Focus Range
Automatic and manual adjustment for accurate zone detection Micromete (micron)

Illumination
Integrated UV and white light illumination (254 nm, 366 nm, 400-700 nm)

Coarse Adjustment Range
Automatic sample height adjustment based on the plate thickness

Fine Adjustment Range
Micrometer-precision Z-axis adjustment for plate scanning

Working Stage
Stainless steel, chemical-resistant plate stage, typically fits 10 x 10 cm or 20 x 10 cm HPTLC plates Square Inch (in2)

Still Image Capture Resolution
Up to 1200 dpi (densitometric scanner capture)

Image Format
16-bit TIFF, JPEG, BMP (exportable from densitometric scanner)

Light Source
High intensity UV and visible light source, long-lasting LED and mercury vapor alternatives

Sample Application
Automated, programmable sample applicator for high precision spotting

Compliance
21 CFR Part 11, GLP, GMP, as per specific system

Plate Material
Silica gel 60 F254, aluminum or glass backed

Weight
Approximately 25 kg (scanner & application module)

Operating Conditions
Ambient temperature 15-30C, humidity <70% RH

Application Type
Qualitative and quantitative analysis of pharmaceuticals, botanicals, food, forensic and environmental samples

Developing Chamber
Automatic humidity and temperature control for reproducible development

Data Management
Integrated software for analysis, reporting, and archiving

Wavelength Range
200 - 800 nm (scan range depending on scanner module)

Power Supply
100-240V AC, 50/60Hz

Detection Method
Densitometry, fluorescence, UV/Vis absorbance, and documentation under visible/UV illumination

High Performance Thin Layer Chromatography (HPTLC) Trade Information

Minimum Order Quantity
1 Number

Supply Ability
5 Per Day

Delivery Time
5 Years

Main Domestic Market
All India

High Performance Thin Layer Chromatography (HPTLC) About

is areputed and reliable brand for HPTLC product line was established in the year1840 by Dr. Peter Desaga who had been working with Prof. Bunsen at theHeidelberg University. In fact, DESAGA was the first company whointroduced the TLC instrumentation in 1957 / 1958 alongwith other accessoriesfor TLC.

Important FieldsOf Application:

1. Herbals:Identification, Stability Test, Detection OfAdulteration, Assay Of Marker Compounds, Etc.

2. Forensics:Detection Of Document Forgery, Investigation Of Poisoning, Dyestuff analyses,Etc.

3. Pharmaceutical Applications: Quality Control,Content Uniformity Test (CUT), Identity-and Purity Checks,

Stability Test, Etc.

4. Food& Feed Stuff: Quality Control, Additives (e.g. Vitamins),Pesticides, Stability Tests (Expiration), Etc.

5. ClinicalApplications: Lipids, Metabolism Studies, Drug Screening, DopingControl, Etc.

6. IndustrialApplications: Process development & Optimization, ProcessMonitoring, Cleaning Validation, Etc.

7.Cosmetics: Identity Of Raw Material, Preservatives, ColoringMaterial, Screening For Illegal Substance, Etc.

8. Environment: Water, Soil, Residue Analysis,Etc.

We have morethan 100 HPTLC installations in India in the field of Forensic Science,Pharmaceuticals, Chemicals, food, Natural & Herbal products,Homeopathic Products, Ayurvedic Products and textile dyes, etc.,

M/s. biostepGmbH, Germany offers following HPTLC System modules and Software :

DESAGA Sample Applicator, Model AS 30 (with built-in display and keyboard)
DESAGA Densitometer-cum-Scanner Model CD 60 for scanning of TLC/HPTLC plates
DESAGA ProQuant 32-bit Software operating under Windows XP/VISTA/7 for quantitative Thin Layer Chromatography
DESAGA Digital Documentation System, Model DD 80
DESAGA Photo Documentation System
DESAGA PC/Software controlled Reagent Sprayer for accurate and precise spraying, Model ChromaJet DS 20
DESAGA TLC-Plate Cutter, Drying Rack, Simultaneous Separating Chambers, Twin-Trough Chambers, Nano-Separating Chambers, H-Separating Chambers, Dipping Chambers
DESAGA TLC Quick Test Set, Carrier Plate with magnetic strips for foils up to 265 x 200 mm
DESAGA IQ / OQ for AS 30, CD 60, DD 80 and ChromaJet DS 20
DESAGA UV Cabinet, Sprayer, Spray Box with Ventilator, Thermo Plate

Some ofthe customers are : Forensic Science Laboratories, CentralDrug Laboratories, Food and Drugs Labs, Ayurvedic Medical College, TextileCommittee, IIT, Agricultural Universities, CSIR Labs and Pharma customers likeDishman Pharmaceuticals, Novartis, Sandoz, Dabur Pharma, IVAX, Apotex, AnimalHusbandries, ICMR Labs, ICAR, Food Hygiene Labs, etc.,

Shouldyou need any technical information, product information, applicationinformation or offer on DESAGA HPTLC System,please do let us know.

If you require any further technicalinformation/clarifications/discussion on above, please contact us.

Automated Sample Application for Precision

HPTLC systems are equipped with programmable, automated sample applicators, ensuring consistent and highly precise sample spotting. This automation reduces human error, enhances reproducibility, and accelerates high-throughput workflows. Laboratory professionals benefit from reliable sample application, enabling them to achieve accurate quantitative results even with minute sample volumes, thus optimizing both efficiency and cost savings in the analysis process.

Accurate and Reproducible Chromatography

With automatic control over humidity and temperature within the developing chamber, HPTLC ensures uniform solvent migration and zone separation. These controls minimize environmental inconsistency, making the development of chromatograms highly reproducible and facilitating reliable results across various applications, including pharmaceuticals, botanicals, and environmental samples.

Advanced Multi-Wavelength Detection and Data Management

The integrated detection system supports densitometry, fluorescence, and UV/Vis absorbance in a scan range from 200 to 800 nm, with image export in multiple formats. Powerful onboard software streamlines analysis, reporting, and data archiving, while compliance with regulatory standards (GLP, GMP, 21 CFR Part 11) ensures secure, professional results suitable for official documentation and audits.

FAQs of High Performance Thin Layer Chromatography (HPTLC):

Q: How does HPTLC improve analytical accuracy compared to traditional TLC?
A: HPTLC offers superior resolution and sensitivity by utilizing fine-particle silica gel plates and automated sample application. The integration of densitometric scanning and programmable environmental controls ensures consistent development and precise quantification, resulting in higher accuracy and reproducibility than conventional TLC methods.

Q: What types of samples can be analyzed using HPTLC?
A: HPTLC is highly versatile and supports the qualitative and quantitative analysis of pharmaceuticals, botanicals, food products, forensic evidence, and environmental samples. Its high sensitivity and selectivity make it suitable for both routine quality control and complex mixture analysis in varied applications.

Q: When should automated sample applicators be used in HPTLC?
A: Automated sample applicators are essential when precise, reproducible sample deposition is needed, especially for quantitative analysis or high-throughput testing. Automation minimizes manual error, ensures even sample distribution, and optimizes comparability between runs, making it ideal for laboratories requiring consistent, high-quality results.

Q: Where is HPTLC typically deployed in India?
A: HPTLC systems are widely used in quality control and research labs across India, particularly in the pharmaceutical, food, botanical, forensic, and environmental sectors. Leading distributors and suppliers provide support, service, and system customization to meet diverse regulatory and analytical requirements across these industries.

Q: What is the process for developing plates in HPTLC systems?
A: Plates are developed within an automated, enclosed chamber that precisely regulates temperature and humidity. This control ensures uniform solvent migration and reproducible separation patterns. Post-development, plates are scanned using a high-sensitivity densitometric scanner, enabling evaluation under multiple wavelengths and lighting conditions.

Q: How does integrated software benefit HPTLC users?
A: Integrated software enables automated analysis, secure archiving, and reporting, simplifying data management and ensuring full compliance with regulatory standards like GLP, GMP, and 21 CFR Part 11. It allows easy transfer, storage, and retrieval of data, facilitating efficient workflow and audit-ready documentation.

Q: What are the advantages of multi-wavelength detection in HPTLC?
A: Multi-wavelength detection enables the analysis of a wide variety of compounds by providing densitometric, fluorescence, and UV/Vis absorbance measurements within a 200800 nm scan range. This flexibility enhances the identification and quantification of different analytes within a single run, increasing throughput and data reliability.