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HPTLC Applicator is highly acknowledged in the market for their high performance and durable in nature. The samples were applied in forms of bands on an aluminum TLC plate pre-coated with silica gel and were separated using dichloromethane: methanol: toluene: ammonia solution as the mobile phase. Further, this applicator are developed and validated for determination of sunitibin malate and possible impurities. Apart from this, HPTLC Applicator is simple in design, inexpensive to construct and ideal for applying small volumes of solutions to TLC strips and plates is described.